Comparative evaluation of UNEX-based DNA extraction for molecular detection of Cyclospora cayetanensis, Toxoplasma gondii, and Cryptosporidium parvum as contaminants of berries
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چکیده
منابع مشابه
Cryptosporidium parvum and Cyclospora cayetanensis: a review of laboratory methods for detection of these waterborne parasites.
Cryptosporidium and Cyclospora are obligate, intracellular, coccidian protozoan parasites that infest the gastrointestinal tract of humans and animals causing severe diarrhea illness. In this paper, we present an overview of the conventional and more novel techniques that are currently available to detect Cryptosporidium and Cyclospora in water. Conventional techniques and new immunological and...
متن کاملToxoplasma gondii and Cryptosporidium parvum lack detectable DNA cytosine methylation.
Epigenetic factors play a role in the expression of virulence traits in Apicomplexa. Apicomplexan genomes encode putative DNA cytosine methylation enzymes. To assess the presence of cytosine methylation of Toxoplasma gondii and Cryptosporidium parvum DNA, we used mass spectrometry analysis and confirmed that these organisms lack detectable methylcytosine in their DNA.
متن کاملPrevalence of infection with Cryptosporidium parvum and Cyclospora cayetanensis among international travellers.
BACKGROUND Cryptosporidium parvum and Cyclospora cayetanensis are recognised as possible pathogens of traveller's diarrhoea. AIMS To identify the prevalence of C parvum and Cyc cayetanensis in travellers returning from developing countries. PATIENTS Nine hundred and seventy eight stool samples wer taken from 795 patients returning from developing countries. METHODS Microscopy (iron-haemat...
متن کاملnano-rods zno as an efficient catalyst for the synthesis of chromene phosphonates, direct amidation and formylation of amines
چکیده ندارد.
Environmental contamination with protozoan parasite infective stages: biology and risk assessment.
In the present paper some aspects of the biology and various factors influencing the potential for environmental contamination with protozoan parasites infective stages implicated in water and foodborne diseases are described. The major protozoan species that affect humans are Entamoeba histolytica, Acanthamoeba sp., Neagleria sp. Giardia intestinalis, Cryptosporidium parvum, Cyclospora cayetan...
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ژورنال
عنوان ژورنال: Food Microbiology
سال: 2020
ISSN: 0740-0020
DOI: 10.1016/j.fm.2020.103447